Brevetoxin / Ciguatoxin Test Kit

Unit Price: $399 Per Kit.
Contact for quote.

Kit Overview

We offer a competitive receptor binding assay kit for the site 5 sodium channel ligands brevetoxin or ciguatoxin.  Brevetoxins and ciguatoxins are produced by marine algae that are then consumed by commercially important shellfish or fish.  When consumed, brevetoxins and ciguatoxins can cause Neurotoxic Shellfish Poisoning (1) or Ciguatera Fish Poisoning (2), respectively.  Our test kit can detect and quantify either of these toxins (3-6). The kit can be used as a screening or quantitative tool with analyses completed in less than 2 hours after extraction. This methodology is faster and less expensive than LC/MS or N2A cell-based methods. Each kit is capable of analyzing from 5 to 8 samples in dilution series (quantitative mode) or up to 40 samples at a single dilution in duplicate (qualitative mode).

References

  1. https://hab.whoi.edu/impacts/impacts-human-health/human-health-neurotoxic-shellfish-poisoning/
  2. https://hab.whoi.edu/impacts/impacts-human-health/human-health-ciguatera-fish-poisoning/
  3. Hardison DR, Holland WC, McCall JR, Bourdelais AJ, Baden DG, Darius HT, Chinain M, Tester PA, Shea D, Flores Quintana HA, Litaker RW. (2016). Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish. PLoS ONE 11(4): article #e0153348. doi: 10.1371/journal.pone.0153348 (OPEN ACCESS)
  4. Litaker RW, Hardison DR, Holland WC, Bourdelais AJ, McCall JR, Baden DG, Morris JA, Bogdanoff AK, Tester PA. (2014). Ciguatoxin concentrations in invasive lionfish estimated using a fluorescent receptor binding assay. 16th ICHA Proceedings. 2014:184-187
  5. McCall JR, Jacocks HM, Niven SC, Poli MA, Baden DG, Bourdelais AJ. (2014). Development and utilization of a fluorescence-based receptor binding assay for site 5 voltage-sensitive sodium channels ligands brevetoxin and ciguatoxin. Journal of the AOAC. 97(2):307-315.
  6. McCall JR, Jacocks HM, Baden DG, Bourdelais AJ. (2012). Development of a competitive fluorescence-based synaptosome binding assay for brevetoxins. Harmful Algae. 19:85-91.

Testing Kits Include:

  • Assay buffer
  • 96-well mixing plate
  • 96-well filter plate
  • Brevetoxin standard (ciguatoxin standard available upon request for an extra fee)
  • Fluorescent ligand
  • Receptor (synaptosomes)
  • Detailed instructions
  • List of supplies to acquire
  • Instrumentation settings are provided

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